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We found that using high-efficiency DNA polymerase was essential for successful DNA amplification, using trace intact plant material as a template DNA source.

View Full-Text ) time (Lanes 1–6 represent 30, 40, 50, 60, 70, and 80 min, respectively) for LAMP analysis using extracted DNA from the leaf of a hermaphrodite plant.

In this study, a molecular technique—based on DNA analysis—was developed for detecting male-hermaphrodite-specific markers to examine the papaya’s sex type.

This method is based on the loop-mediated isothermal amplification (LAMP) and does not require prior DNA purification.

If you think that you’re suffering sex discrimination at work, you should talk to your employer, explaining what you see as discrimination. An employee representative, like a trades union official, may be able to help you..pass_color_to_child_links a.u-inline.u-margin-left--xs.u-margin-right--sm.u-padding-left--xs.u-padding-right--xs.u-relative.u-absolute.u-absolute--center.u-width--100.u-flex-inline.u-flex-align-self--center.u-flex-justify--between.u-serif-font-main--regular.js-wf-loaded .u-serif-font-main--regular.amp-page .u-serif-font-main--regular.u-border-radius--ellipse.u-hover-bg--black-transparent.web_page .u-hover-bg--black-transparent:hover. Content Header .feed_item_answer_user.js-wf-loaded . LAMP analysis, loop-mediated isothermal amplification (LAMP) analysis; M, mark.

" Figure 4 Transferability of six different papaya varieties using the LAMP assay without prior DNA purification detected by agarose gel electrophoresis. Wiley Online Library requires cookies for authentication and use of other site features; therefore, cookies must be enabled to browse the site.